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Product details

Annexin V-APC/Cyanine7/PI Apoptosis Kit

Catalog number E-CK-A229-01

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Price for E-CK-A229-01

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Size / count 20 Assays

Specifications

Catalog number
E-CK-A229-01
Size / count
20 Assays
Dry ice
No
Hazardous
No
Discontinued
No
Detection Method
Fluorometric method; Flow cytometry
Assay Principle
Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.
Sample Type
Cell samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2-8°C for 12 months with shading light.
Citation 01
https://www.nature.com/articles/s41467-024-55557-4
Citation 02
https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.202410214
Citation 03
https://pubs.acs.org/doi/abs/10.1021/acsami.4c18469
Citation 04
https://www.sciencedirect.com/science/article/pii/S0753332224011041
Citation 05
https://www.mdpi.com/1999-4923/16/2/224
Citation 06
https://pmc.ncbi.nlm.nih.gov/articles/PMC12093249/
Detection Instrument
Flow Cytometer

Frequently Asked Questions

What is the catalog number for Annexin V-APC/Cyanine7/PI Apoptosis Kit?

The catalog number for Annexin V-APC/Cyanine7/PI Apoptosis Kit is E-CK-A229.

What sizes are available for Annexin V-APC/Cyanine7/PI Apoptosis Kit?

Annexin V-APC/Cyanine7/PI Apoptosis Kit is available in the following size(s): 20 Assays, 100 Assays.

How many pack sizes does Annexin V-APC/Cyanine7/PI Apoptosis Kit come in?

Annexin V-APC/Cyanine7/PI Apoptosis Kit is available in 2 different pack sizes.

Is Annexin V-APC/Cyanine7/PI Apoptosis Kit in stock?

Annexin V-APC/Cyanine7/PI Apoptosis Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Annexin V-APC/Cyanine7/PI Apoptosis Kit?

Annexin V-APC/Cyanine7/PI Apoptosis Kit is manufactured by Elabscience.

What is the estimated delivery time for Annexin V-APC/Cyanine7/PI Apoptosis Kit?

Estimated delivery time for Annexin V-APC/Cyanine7/PI Apoptosis Kit is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Annexin V-APC/Cyanine7/PI Apoptosis Kit?

The safety data sheet for Annexin V-APC/Cyanine7/PI Apoptosis Kit is available here: https://789.bio/eb/GC4mbP.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

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