Mitochondrial Membrane Potential Assay Kit (with JC-1)

Specifications

Catalog number

E-CK-A301-01

Size / count

20 Assays

Dry ice

No

Hazardous

No

Discontinued

No

Detection Method

Fluorometric method; Flow cytometry

Assay Principle

The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic features (chromatin condensation and DNA fragmentation) . Once the mitochondrial membrane potential collapses, apoptosis is irreversible. JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ?Ψm. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t accumulate in the mitochondrial matrix. When JC-1 is a monomer, it can produce green fluorescence. The decrease of cell membrane potential can be detected by the transition of JC-1 from red fluorescence to green fluorescence, and the transition of JC-1 fluorescence color can be used as an early detection indicator of cell apoptosis. The relative ratio of red and green fluorescence is commonly used to measure the ratio of mitochondrial depolarization. The maximum excitation wavelength of JC-1 monomer is 514 nm and the maximum emission wavelength is 529 nm; the maximum excitation wavelength of JC-1 polymer is 585 nm and the maximum emission wavelength is 590 nm.

Sample Type

Cell samples

Shipping Conditions

Ice bag

Storage Conditions

This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

Citation 01

https://www.sciencedirect.com/science/article/pii/S2452199X23003316

Citation 02

https://www.sciencedirect.com/science/article/pii/S1385894724031966

Citation 03

https://www.sciencedirect.com/science/article/pii/S174801322400450X

Citation 04

https://www.sciencedirect.com/science/article/pii/S1385894725040793

Citation 05

https://www.sciencedirect.com/science/article/pii/S0142961224000085

Citation 06

https://www.sciencedirect.com/science/article/pii/S2090123225000293

Citation 07

https://jeccr.biomedcentral.com/articles/10.1186/s13046-023-02713-1

Citation 08

https://link.springer.com/article/10.1186/s12951-023-02185-7

Citation 09

https://onlinelibrary.wiley.com/doi/abs/10.1002/adhm.202400770

Citation 10

https://www.sciencedirect.com/science/article/pii/S0168365924005789

Citation 11

https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-024-02876-9

Citation 12

https://pubs.acs.org/doi/abs/10.1021/acsami.3c19621

Detection Instrument

Flow Cytometer; Fluorescence Microscope