E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488)

Catalog number E-CK-A376-01

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Price for E-CK-A376-01

Size / count 50 Assays

Specifications

Catalog number: E-CK-A376-01
Size / count: 50 Assays
Dry ice: No
Hazardous: No
Discontinued: No
Detection Method: Fluorometric method
Assay Principle: Cell proliferation assays are widely used in the evaluation of cell viability, genotoxicity, and the effect of antitumor drugs. Direct detection of DNA synthesis in cells is considered to be the most accurate method for detecting cell proliferation. The initial widely used method for detecting DNA synthesis in cells was the radiolabeled nucleoside incorporation method, but this method was greatly limited due to radioactive contamination and the difficulty of single-cell detection, and was gradually replaced by the BrdU method based on antibody detection. The BrdU method has many steps and requires the use of BrdU antibody, which has many influencing factors and poor stability. EdU method is based on EdU incorporation and subsequent click reaction, without the use of antibodies, convenient operation and high detection sensitivity. It is a new method upgraded on the basis of BrdU method and will gradually replace BrdU method. EdU (5-ethynyl-2-deoxyuridine), is a thymidine analog, EdU can replace thymidine in the process of DNA synthesis to incorporate into new in synthetic DNA. On the other hand, the acetylene group on EdU can react with fluorescently labeled small molecule azide probes (such as FITC Azide, Elab Fluor? 488 Azide, Elab Fluor? 594 Azide, Elab Fluor? 647 Azide) through the catalysis of monovalent copper ions to form a stable triazole ring. This reaction is very rapid and is called the click reaction. Through the click reaction, the newly synthesized DNA is labeled with the corresponding fluorescent probe, so that the proliferating cells can be detected using the appropriate fluorescent detection equipment.
Sample Type: Cell Slides; Cell Smears
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.
Citation 01: https://www.sciencedirect.com/science/article/pii/S0944711325006531
Citation 02: https://onlinelibrary.wiley.com/doi/abs/10.1002/ctm2.1742
Citation 03: https://www.mdpi.com/2076-3921/13/7/875
Citation 04: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-024-05613-w
Citation 05: https://www.sciencedirect.com/science/article/pii/S094471132400984X
Citation 06: https://link.springer.com/article/10.1186/s13148-024-01769-w
Citation 07: https://www.mdpi.com/2227-9059/11/6/1524
Citation 08: https://www.sciencedirect.com/science/article/pii/S1567576925010525
Citation 09: https://link.springer.com/article/10.1186/s12950-025-00444-y
Citation 10: https://www.sciencedirect.com/science/article/pii/S0898656825001548
Citation 11: https://www.sciencedirect.com/science/article/pii/S0014483525001095
Citation 12: https://www.nature.com/articles/s41598-025-94968-1
Detection Instrument: Fluorescence Microscope

Frequently Asked Questions

What is the catalog number for E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488)?

The catalog number for E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) is E-CK-A376.

What sizes are available for E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488)?

E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) is available in the following size(s): 50 Assays, 200 Assays.

How many pack sizes does E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) come in?

E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) is available in 2 different pack sizes.

Is E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) in stock?

E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488)?

E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) is manufactured by Elabscience.

What is the estimated delivery time for E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488)?

Estimated delivery time for E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488)?

The safety data sheet for E-Click EdU Cell Proliferation Imaging Assay Kit (Green, Elab Fluor® 488) is available here: https://789.bio/eb/bbrDK0.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

What temperature does this product ship at?

Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.