E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594)

Catalog number E-CK-A377-01

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Price for E-CK-A377-01

Size / count 50 Assays

Specifications

Catalog number: E-CK-A377-01
Size / count: 50 Assays
Dry ice: No
Hazardous: No
Discontinued: No
Detection Method: Fluorometric method
Assay Principle: Cell proliferation assays are widely used in the evaluation of cell viability, genotoxicity, and the effect of antitumor drugs. Direct detection of DNA synthesis in cells is considered to be the most accurate method for detecting cell proliferation. The initial widely used method for detecting DNA synthesis in cells was the radiolabeled nucleoside incorporation method, but this method was greatly limited due to radioactive contamination and the difficulty of single-cell detection, and was gradually replaced by the BrdU method based on antibody detection. The BrdU method has many steps and requires the use of BrdU antibody, which has many influencing factors and poor stability. EdU method is based on EdU incorporation and subsequent click reaction, without the use of antibodies, convenient operation and high detection sensitivity. It is a new method upgraded on the basis of BrdU method and will gradually replace BrdU method. EdU (5-ethynyl-2-deoxyuridine), is a thymidine analog, EdU can replace thymidine in the process of DNA synthesis to incorporate into new in synthetic DNA. On the other hand, the acetylene group on EdU can react with fluorescently labeled small molecule azide probes (such as FITC Azide, Elab Fluor? 488 Azide, Elab Fluor? 594 Azide, Elab Fluor? 647 Azide) through the catalysis of monovalent copper ions to form a stable triazole ring. This reaction is very rapid and is called the click reaction. Through the click reaction, the newly synthesized DNA is labeled with the corresponding fluorescent probe, so that the proliferating cells can be detected using the appropriate fluorescent detection equipment.
Sample Type: Cell Slides; Cell Smears
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.
Citation 01: https://link.springer.com/article/10.1186/s12951-025-03450-7
Citation 02: https://link.springer.com/article/10.1007/s10456-025-09994-4
Citation 03: https://www.sciencedirect.com/science/article/pii/S026974912301093X
Citation 04: https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202402619R
Citation 05: https://onlinelibrary.wiley.com/doi/abs/10.1111/cas.16193
Citation 06: https://pmc.ncbi.nlm.nih.gov/articles/PMC11568593/
Citation 07: https://onlinelibrary.wiley.com/doi/abs/10.1002/fsn3.4360
Citation 08: https://www.nature.com/articles/s41598-025-96038-y
Citation 09: https://pmc.ncbi.nlm.nih.gov/articles/PMC12210165/
Detection Instrument: Fluorescence Microscope

Frequently Asked Questions

What is the catalog number for E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594)?

The catalog number for E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) is E-CK-A377.

What sizes are available for E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594)?

E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) is available in the following size(s): 50 Assays, 200 Assays.

How many pack sizes does E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) come in?

E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) is available in 2 different pack sizes.

Is E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) in stock?

E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594)?

E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) is manufactured by Elabscience.

What is the estimated delivery time for E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594)?

Estimated delivery time for E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594)?

The safety data sheet for E-Click EdU Cell Proliferation Imaging Assay Kit (Red, Elab Fluor® 594) is available here: https://789.bio/eb/vL4CeH.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.