Caspase 3/7 Activity Assay Kit (Colorimetric Method)

Catalog number E-CK-A383-01

Choose a variant (2 options)

Selecting a variant updates its price, size and availability.

Price for E-CK-A383-01

Size / count 20 Assays

Specifications

Catalog number: E-CK-A383-01
Size / count: 20 Assays
Dry ice: No
Hazardous: No
Discontinued: No
Detection Method: Colorimetric method
Assay Principle: Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.
Sample Type: Cell and tissue samples
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.
Citation 01: https://pubs.acs.org/doi/abs/10.1021/acs.est.4c10984
Citation 02: https://www.tandfonline.com/doi/abs/10.2147/IJN.S417928
Citation 03: https://www.sciencedirect.com/science/article/pii/S0925521423003162
Citation 04: https://www.sciencedirect.com/science/article/pii/S0925521423001369
Citation 05: https://www.sciencedirect.com/science/article/pii/S0141813025014394
Citation 06: https://www.sciencedirect.com/science/article/pii/S0141813025006841
Citation 07: https://www.sciencedirect.com/science/article/pii/S0141813024082667
Citation 08: https://www.sciencedirect.com/science/article/pii/S0141813025035822
Citation 09: https://www.sciencedirect.com/science/article/pii/S0141813025042424
Citation 10: https://www.mdpi.com/2073-4409/12/23/2734
Citation 11: https://www.mdpi.com/2072-6643/14/21/4605
Citation 12: https://www.sciencedirect.com/science/article/pii/S0925521422002678
Detection Instrument: Spectrophotometer; Microplate reader

Frequently Asked Questions

What is the catalog number for Caspase 3/7 Activity Assay Kit (Colorimetric Method)?

The catalog number for Caspase 3/7 Activity Assay Kit (Colorimetric Method) is E-CK-A383.

What sizes are available for Caspase 3/7 Activity Assay Kit (Colorimetric Method)?

Caspase 3/7 Activity Assay Kit (Colorimetric Method) is available in the following size(s): 20 Assays, 100 Assays.

How many pack sizes does Caspase 3/7 Activity Assay Kit (Colorimetric Method) come in?

Caspase 3/7 Activity Assay Kit (Colorimetric Method) is available in 2 different pack sizes.

Is Caspase 3/7 Activity Assay Kit (Colorimetric Method) in stock?

Caspase 3/7 Activity Assay Kit (Colorimetric Method) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Caspase 3/7 Activity Assay Kit (Colorimetric Method)?

Caspase 3/7 Activity Assay Kit (Colorimetric Method) is manufactured by Elabscience.

What is the estimated delivery time for Caspase 3/7 Activity Assay Kit (Colorimetric Method)?

Estimated delivery time for Caspase 3/7 Activity Assay Kit (Colorimetric Method) is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Caspase 3/7 Activity Assay Kit (Colorimetric Method)?

The safety data sheet for Caspase 3/7 Activity Assay Kit (Colorimetric Method) is available here: https://789.bio/eb/u9G4i5.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.