Caspase 9 Activity Assay Kit (Colorimetric Method)

Catalog number E-CK-A389-01

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Price for E-CK-A389-01

Size / count 20 Assays

Specifications

Catalog number: E-CK-A389-01
Size / count: 20 Assays
Dry ice: No
Hazardous: No
Discontinued: No
Detection Method: Colorimetric method
Assay Principle: Caspase 9, also known as ICE-LAP6 or Mch6, is a relatively upstream caspase in the process of apoptosis signal transduction. After mitochondria release cytochrome c, caspase 9 can form complex with cytochrome c and Apaf1 and can be activated. Activated caspase 9 can activate caspase 3, the most critical enzyme of apoptosis, thereby promoting subsequent apoptotic signals. The activation of caspase 9 can be regulated by phosphorylation. This caspase 9 activity assay kit is based on caspase 9 that can catalyze the substrate Ac-LEHD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 9 can be calculated by determining the absorbance at 405 nm.
Sample Type: Cell and tissue samples
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.
Citation 01: https://www.sciencedirect.com/science/article/pii/S0141813025006841
Citation 02: https://www.sciencedirect.com/science/article/pii/S0925521423003162
Citation 03: https://www.sciencedirect.com/science/article/pii/S0925521423001369
Citation 04: https://www.sciencedirect.com/science/article/pii/S0925521422002678
Citation 05: https://www.sciencedirect.com/science/article/pii/S0925521424005106
Citation 06: https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c09673
Citation 07: https://www.sciencedirect.com/science/article/pii/S1773224724008281
Citation 08: https://link.springer.com/article/10.1007/s12035-025-04810-y
Citation 09: https://www.nature.com/articles/s41598-025-96788-9
Citation 10: https://link.springer.com/article/10.1007/s00210-025-04355-w
Citation 11: https://www.sciencedirect.com/science/article/pii/S1756464624001713
Citation 12: https://www.sciencedirect.com/science/article/pii/S0039128X22001908
Detection Instrument: Spectrophotometer; Microplate reader

Frequently Asked Questions

What is the catalog number for Caspase 9 Activity Assay Kit (Colorimetric Method)?

The catalog number for Caspase 9 Activity Assay Kit (Colorimetric Method) is E-CK-A389.

What sizes are available for Caspase 9 Activity Assay Kit (Colorimetric Method)?

Caspase 9 Activity Assay Kit (Colorimetric Method) is available in the following size(s): 20 Assays, 100 Assays.

How many pack sizes does Caspase 9 Activity Assay Kit (Colorimetric Method) come in?

Caspase 9 Activity Assay Kit (Colorimetric Method) is available in 2 different pack sizes.

Is Caspase 9 Activity Assay Kit (Colorimetric Method) in stock?

Caspase 9 Activity Assay Kit (Colorimetric Method) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Caspase 9 Activity Assay Kit (Colorimetric Method)?

Caspase 9 Activity Assay Kit (Colorimetric Method) is manufactured by Elabscience.

What is the estimated delivery time for Caspase 9 Activity Assay Kit (Colorimetric Method)?

Estimated delivery time for Caspase 9 Activity Assay Kit (Colorimetric Method) is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Caspase 9 Activity Assay Kit (Colorimetric Method)?

The safety data sheet for Caspase 9 Activity Assay Kit (Colorimetric Method) is available here: https://789.bio/eb/DC0iPC.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.