One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) — image 1 of 2

Product details

One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)

Catalog number E-CK-A421-01

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Selecting a variant updates its price, size and availability.

Price for E-CK-A421-01

Size / count 20 Assays

Specifications

Catalog number: E-CK-A421-01
Size / count: 20 Assays
Dry ice: No
Hazardous: No
Discontinued: No
Detection Method: Fluorometric method; Flow cytometry
Assay Principle: When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.
Sample Type: Cell samples
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 12 months with shading light.
Citation 01: https://www.sciencedirect.com/science/article/pii/S0024320524009147
Citation 02: https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.12002
Detection Instrument: Flow Cytometer

Frequently Asked Questions

What is the catalog number for One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)?

The catalog number for One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) is E-CK-A421.

What sizes are available for One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)?

One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) is available in the following size(s): 20 Assays, 100 Assays.

How many pack sizes does One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) come in?

One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) is available in 2 different pack sizes.

Is One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) in stock?

One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)?

One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) is manufactured by Elabscience.

What is the estimated delivery time for One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)?

Estimated delivery time for One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)?

The safety data sheet for One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488) is available here: https://789.bio/eb/W5OiLO.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.