Phospho-IkB alpha (S32/S36) Recombinant Rabbit Monoclonal Antibody [PSH06-76]

About this product

IκBα (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha; NFKBIA) is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. IκBα inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, IκBα blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

Specifications

Catalog number

HA722770

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

I kappa B alpha antibody I-kappa-B-alpha antibody IkappaBalpha antibody IkB-alpha antibody IKBA antibody IKBA_HUMAN antibody IKBalpha antibody MAD 3 antibody MAD3 antibody Major histocompatibility complex enhancer-binding protein MAD3 antibody NF kappa B inhibitor alpha antibody NF-kappa-B inhibitor alpha antibody NFKBI antibody NFKBIA antibody Nuclear factor of kappa light chain gene enhancer in B cells antibody Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

Abbreviation

NF-kappa-B inhibitor alpha I-kappa-B-alpha (IkB-alpha; IkappaBalpha) Major histocompatibility complex enhancer-binding protein MAD3 NFKBIA IKBA, MAD3, NFKBI

Swiss Prot

P25963 Human, Q9Z1E3 Mouse, Q63746 Rat

Cellular Locus

Cytoplasm, Nucleus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser32/Ser36 of human IkB alpha aa 1-50.

Isotype

IgG

Clone

PSH06-76

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell

Positive Control

HeLa treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes cell lysate, NIH/3T3 treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes cell lysate, C6 treated with 100ng/mL Calyculin A for 1 hours cell lysate, human breast cancer tissue, HeLa cells treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes, NIH/3T3 cells treated with 20ng/mL TNF-α and 100nM Calyculin A for 10 minutes.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IHC-P: 1:200 ;IF-Cell: 1:100-1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 36 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes