Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71]

Catalog number HA723023

Price for HA723023

Size / count 100 µL

About this product

Protein phosphatase 1 regulatory subunit 12A is an enzyme that in humans is encoded by the PPP1R12A gene. Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.

Specifications

Catalog number: HA723023
Size / count: 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9000-83-3
Product Name Alternative: M130 antibody MBS antibody Myosin binding subunit antibody Myosin phosphatase target subunit 1 antibody Myosin phosphatase targeting subunit 1 antibody Myosin phosphatase-targeting subunit 1 antibody MYPT1 antibody MYPT1_HUMAN antibody PPP1R12A antibody Protein phosphatase 1 regulatory inhibitor subunit 12A antibody Protein phosphatase 1 regulatory subunit 12A antibody Protein phosphatase 1, regulatory (inhibitor) subunit 12A antibody Protein phosphatase myosin binding subunit antibody Protein phosphatase myosin-binding subunit antibody
Abbreviation: Protein phosphatase 1 regulatory subunit 12A Myosin phosphatase-targeting subunit 1 (Myosin phosphatase target subunit 1) Protein phosphatase myosin-binding subunit PPP1R12A MBS MYPT1
Swiss Prot: O14974 Human, Q9DBR7 Mouse, Q10728 Rat
Cellular Locus: Cytoplasm, cytoskeleton, stress fiber.
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr853 of Human MYPT1.
Isotype: IgG
Clone: PSH08-71
Conjugation: Non-conjugated
Type: Recombinant Rabbit monoclonal Antibody
Applications: WB
Positive Control: 293T cell lysate, HeLa cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, C6 cell lysate.
Concentration: 1 mg/mL
Dilution: WB: 1:2,000
Purity: Protein A affinity purified.
Form: Liquid
Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight: Predicted band size: 115 kDa
Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody: Yes

Frequently Asked Questions

What is the catalog number for Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71]?

The catalog number for Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71] is HA723023.

What sizes are available for Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71]?

Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71] is available in the following size(s): 100 µL.

Is Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71] in stock?

Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71] is currently made to order / not held in immediate stock. Contact us for lead time.

What is Phospho-MYPT1 (T853) Recombinant Rabbit Monoclonal Antibody [PSH08-71] used for?

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.