Uncoated Human MCP-4 (Monocyte Chemotactic Protein 4) ELISA Kit

Specifications

Catalog number

E-UNEL-H0378-01

Size / count

5x 96 Tests

Dry ice

No

Hazardous

No

Discontinued

No

Abbreviation

MCP-4

UniProt

Q99616

Cross Reactivity

This kit recognizes MCP-4 in samples.No significant cross-reactivity or interference between MCP-4 and analogues was observed.

Target

SCYA13; MCP-4; NCC-1; SCYL1; CKb10; CCL13; Monocyte chemeotactic protein 4; MCP4; CKβ10; New CC chemokine 1; NCC1; Small inducible cytokine subfamily a; member 13

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Cell Biology; Signal Transduction

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MCP-4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MCP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MCP-4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MCP-4 . You can calculate the concentration of MCP-4 in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

24 h

Sample Type

Serum, plasma and other biological fluids

Detection Range

78.13-5000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%

Recovery

80%-120%

Shipping Conditions

Ice bag

Storage Conditions

-20°C,12 months

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human MCP-4 concentrations in serum, plasma and other biological fluids.