Recombinant Human TNFRSF11B Protein (His Tag)

Specifications

Catalog number

PDMH100166-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Osteoprotegerin or TNFRSF11B is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. Osteoprotegerin/TNFRSF11B acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. This protein may inhibit the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. Osteoprotegerin/TNFRSF11B also play a role in preventing arterial calcification, act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

Abbreviation

TNFRSF11B

Synonyms

PDB; MGC; TNFRSF11B; OCIF; OPG; PDB5; TR1; MGC29565; TNF receptor superfamily member 11b; TNFRSF 11B; TR 1; TR11B; Osteoprotegerin; Tumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; TNFRSF11B; TR1

UniProt

O00300

Accession Number

O00300

Expression System

Mammalian

Tag

C-His

Sequence

Met1-Leu401

Field of Research

Cancer; Cardiovascular; Cell Biology; Immunology

Endotoxin

< 1.0 EU/mg of the protein as determined by the LAL method

Purity

> 95% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44 kDa

Observed Molecular Weight

58 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human