CD100 (Semaphorin-4D) (A8), CF647 conjugate, 0.1mg/mL

About this product

CD100 (also known as Semaphorin 4D), is a homodimeric protein comprised of 50 kDa subunits. It is expressed on resting T cells and weakly on other lymphocytes, and its expression is increased on B and T cells after stimulation. CD100 is a cell surface receptor that binds to CD72, Plexin B1 and Plexin B2. It promotes B cell aggregation, cell survival, and cell proliferation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470218-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD100; Semaphorin-4D; SEMA4D

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

SEMA4D

Gene ID

10507

NCBI Gene ID

494406

UniProt

Q92854

Swiss Prot

Q92854

Host

Mouse

Species Reactivity

Human, Monkey, Mouse

Reactivity

CD100|Semaphorin-4D

Immunogen

PHA stimulated human peripheral blood lymphocytes.

Target Antigen

CD100 | Semaphorin-4D

Clonality

Monoclonal

Clone

A8

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |ELISA (published) |WB (published)

Field of Research

Immunology, Neuroscience, Signal transduction

Positive Control

Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

50 kDa (Monomer)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1996) 93:11780-11785. (Flow) PLoS One (2007) 2(9):e818. (Flow, WB, ELISA)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647