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Product details
Catalog number BNC743518-500
Price for BNC743518-500
Size / count 1x 500 µL
Please note that this antibody is a recombinant Mouse version of original anti-histone H1 antibody (Clone AE-4) . Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a purer preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
The catalog number for Histone H1 (Pan Nuclear Marker) (rAE-4), CF740 conjugate, 0.1mg/mL is BNC743518-500.
Histone H1 (Pan Nuclear Marker) (rAE-4), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.
Histone H1 (Pan Nuclear Marker) (rAE-4), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.
Histone H1 (Pan Nuclear Marker) (rAE-4), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.
Estimated delivery time for Histone H1 (Pan Nuclear Marker) (rAE-4), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.
The safety data sheet for Histone H1 (Pan Nuclear Marker) (rAE-4), CF740 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.
Please note that this antibody is a recombinant Mouse version of original anti-histone H1 antibody (Clone AE-4) . Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of
A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.
Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.
Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.
