Mitofilin Recombinant Rabbit Monoclonal Antibody [PSH01-18]

About this product

Mitochondrial inner membrane protein is a protein that in humans is encoded by the IMMT gene. IMMT encodes an inner mitochondrial membrane (IMM) protein in the nucleus. It is posttranslational transported to the IMM. Mic60/Mitofilin (encoded by the IMMT gene) is a core subunit of the MICOS-complex, directly located next to cristae junctions (CJ). Human Mic60 exists in two isoforms of different size, anchored to the IMM via its N-terminus, while most of the protein is located to the inner mitochondrial space (IMS). Mic60 is evolutionary one of the oldest MICOS subunits as homologous were found in anaerobic prokaryotes. It is mainly present in two isoforms (ca. 88 and 90 kDa). In the brain, four isoforms are known, which differ in their isoelectric point due to different post-translational modifications. The amino terminus of Mic60 is anchored in the IM, while most of the protein is extended to the IMS. C-terminal Mic60 has a conserved mitofilin domain which is crucial for building the MICOS-complex. A central coiled-coil domain is required to enable protein-protein interactions.

Specifications

Catalog number

HA721635

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

Cell proliferation inducing protein 52 antibody Cell proliferation-inducing gene 4/52 protein antibody Heart muscle protein antibody HMP antibody IMMT antibody IMMT_HUMAN antibody Inner membrane protein mitochondrial antibody Inner mitochrondial membrane antibody MICOS complex subunit MIC60 antibody MINOS2 antibody Mitochondrial inner membrane organizing system 2 antibody Mitochondrial inner membrane protein antibody Mitofilin antibody Motor protein antibody p87 antibody p87/89 antibody p89 antibody pig4 antibody PIG52 antibody Proliferation-inducing gene 4 antibody

Abbreviation

IMMT, HMP, MIC60, MINOS2

Swiss Prot

Q16891 Human, Q8CAQ8 Mouse, Q3KR86 Rat

Cellular Locus

Mitochondrion inner membrane, Mitochondrion.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within human Mitofilin aa 151-400 / 758.

Isotype

IgG

Clone

PSH01-18

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P, IF-Cell, IF-Tissue

Positive Control

HeLa cell lysate, Raji cell lysate, HEK-293 cell lysate, HepG2 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human pancreas tissue, human thyroid carcinoma tissue, mouse brain tissue, rat brain tissue, HeLa.

Concentration

1 mg/mL

Dilution

WB: 1:5,000 ;IHC-P: 1:1,000-1:5,000 ;IF-Cell: 1:500 ;IF-Tissue: 1:200

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 84 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes