HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL — image 1 of 2

Product details

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL

Catalog number BNC742308-500

Price for BNC742308-500

Size / count 1x 500 µL

About this product

MAb rHSPB1/774 reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha-crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumorsPrimary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC742308-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Heat shock 27kDa protein, Estrogen-regulated 24kDa protein, Heat shock 25kDa protein 1, Heat shock 28kDa protein 1, Heat shock protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive protein 27 (SRP27)
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: HSPB1
Gene ID: 3315
NCBI Gene ID: 520973
UniProt: P04792
Swiss Prot: P04792
Host: Mouse
Species Reactivity: Chicken, Chimpanzee, Human, Monkey, Mouse, Rat, Sheep
Reactivity: HSP27
Immunogen: Recombinant full-length human HSPB1 protein
Target Antigen: HSP27
Clonality: Recombinant Monoclonal
Clone: RHSPB1/774
Type: Primary
Source: Animal
Origin: Animal
Field of Research: Endocrinology, Heat shock proteins
Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% rBSA, 0.05% azide
Molecular Weight: 27 kDa
References & Citations: Edwards DP et. al. Biochem Biophys Research Commun, 93:804-812, 1980. |Ciocca DR et. al. Breast Cancer Research and Treatment, 20:33-42, 199
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), Bos taurus (bovine)
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF740

Frequently Asked Questions

What is the catalog number for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL?

The catalog number for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL is BNC742308-500.

What sizes are available for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL?

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL in stock?

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL?

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL?

Estimated delivery time for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL?

The safety data sheet for HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF740 conjugate, 0.1mg/mL used for?

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.