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Product details

BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL

Catalog number BNUB0522-500

Price for BNUB0522-500

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Size / count 1x 500 µL

About this product

Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL) .

Specifications

Catalog number
BNUB0522-500
Size / count
1x 500 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
Bromodeoxyuridine; 5-bromo-2-deoxyuridine
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
Not Applicable
UniProt
Not Applicable
Swiss Prot
Not Applicable
Host
Mouse
Species Reactivity
Pan species
Reactivity
BrdU
Immunogen
Bromodeoxyuridine (BrdU) conjugated to KLH
Target Antigen
BrdU
Clonality
Monoclonal
Clone
BU20a
Type
Primary
Source
Animal
Origin
Animal
Applications
IHC, FFPE (verified)
Field of Research
Cancer, Cell cycle
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU
Stability
2 years
Concentration
0.2 mg/mL
Buffer
PBS, 0.05% BSA, 0.05% azide
Molecular Weight
Depends on the target
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG1
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate
Purified, with BSA

Frequently Asked Questions

What is the catalog number for BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL?

The catalog number for BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL is BNUB0522-500.

What sizes are available for BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL?

BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL is available in the following size(s): 1x 500 µL.

Is BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL in stock?

BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL?

BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL?

Estimated delivery time for BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL?

The safety data sheet for BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is BrdU (Bromodeoxyuridine) (BU20a), 0.2mg/mL used for?

Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DN

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

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