Calgranulin B / MRP-8/-14 / MAC 387 / S100A8/A9 / Macrophage Ag (MAC387), CF568 conjugate, 0.1mg/mL

About this product

Calprotectin is a heteroduplex of Protein S100-A8/A9 (also called MRP-8/14, or Calgranulin A/B) . This antibody recognizes the 14 kDa B component (Protein S100-A9) of the complex. Protein S100-A9 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. Altered expression of this protein is associated with the disease cystic fibrosis. This antibody reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC680035-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Calprotectin; Protein S100-A9; Myeloid-related protein 14; MRP-14; Calgranulin B; CAGB; Calprotectin L1H Subunit

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene ID

6279 (S100A8/Calgranulin A/MRP-8) & 6280 (S100A9/Calgranulin B/MRP-14)

NCBI Gene ID

416073 (S100A8/Calgranulin A/MRP-8) & 112405 (S100A9/Calgranulin B/MRP-14)

UniProt

P05109 (S100A8/Calgranulin A/MRP-8); P06702 (S100A9/Calgranulin B/MRP-14)

Swiss Prot

P05109 (S100A8/Calgranulin A/MRP-8) & P06702 (S100A9/Calgranulin B/MRP-14)

Host

Mouse

Species Reactivity

Baboon, Cat, Cow, Dog, Goat, Guinea pig, Horse, Human, Monkey, Mouse, Pig, Rabbit, Rat

Reactivity

Calprotectin|S100-A9

Immunogen

Affinity purified monocyte membrane preparation

Target Antigen

Calprotectin | S100-A9

Clonality

Monoclonal

Clone

MAC387

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular (published) |IHC, FFPE (verified) |IP (published) |WB (published)

Field of Research

Immunology, Inflammation

Positive Control

Tonsil, lymph node, or spleen

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

12-14 kDa (doublet)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Histochem Cytochem (1987) 35(11): 1217-1226 (IP; WB; IHC, FFPE) J Histochem Cytochem (1990) 38(11): 1669-1675. (IHC, FFPE) J Leukocyte Biol (1994) 55: 259. (WB; IHC) World J Gastroenterol (2017)23(8): 1477-1488. (Flow, intracellular)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF568