EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL — image 1 of 2

Product details

EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL

Catalog number BNC881176-100

Price for BNC881176-100

Size / count 1x 100 µL

About this product

This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1) . It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC881176-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: Early Mitotic Inhibitor-1 (EMI1) ; FBX5; Fbxo31
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: FBXO5
Gene ID: 26271
NCBI Gene ID: 520506
UniProt: Q9UKT4
Swiss Prot: Q9UKT4
Host: Mouse
Species Reactivity: Human
Reactivity: EMI1
Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
Target Antigen: EMI1
Clonality: Monoclonal
Clone: EMI1/1176
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified) |WB (verified)
Field of Research: Cell cycle
Positive Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 56 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF488A

Frequently Asked Questions

What is the catalog number for EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL?

The catalog number for EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL is BNC881176-100.

What sizes are available for EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL?

EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL in stock?

EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL?

EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL?

Estimated delivery time for EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL?

The safety data sheet for EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is EMI1 (EMI1/1176), CF488A conjugate, 0.1mg/mL used for?

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.