CD16 (C16/1045), Biotin conjugate, 0.1mg/mL

Catalog number BNCB1045-100

Price for BNCB1045-100

Size / count 1x 100 µL

About this product

This antibody recognizes CD16 (FcÎ3RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (FcÎ3RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcÎ3RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB1045-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CD162; CLA; Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; PSGL1; Selectin P ligand; SELPLG
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: FCGR3A
Gene ID: 2214
NCBI Gene ID: 372679
UniProt: P08637
Swiss Prot: P08637
Host: Mouse
Species Reactivity: Human
Reactivity: CD16|Fc gamma receptor III
Immunogen: Recombinant human CD16 protein
Target Antigen: CD16 | Fc gamma receptor III
Clonality: Monoclonal
Clone: C16/1045
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, surface (verified) |IF (verified)
Field of Research: Immunology
Positive Control: K-562 or U-937 cells. Lymph nodes and tonsils.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 50-80 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989.
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for CD16 (C16/1045), Biotin conjugate, 0.1mg/mL?

The catalog number for CD16 (C16/1045), Biotin conjugate, 0.1mg/mL is BNCB1045-100.

What sizes are available for CD16 (C16/1045), Biotin conjugate, 0.1mg/mL?

CD16 (C16/1045), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD16 (C16/1045), Biotin conjugate, 0.1mg/mL in stock?

CD16 (C16/1045), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD16 (C16/1045), Biotin conjugate, 0.1mg/mL?

CD16 (C16/1045), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD16 (C16/1045), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for CD16 (C16/1045), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD16 (C16/1045), Biotin conjugate, 0.1mg/mL?

The safety data sheet for CD16 (C16/1045), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD16 (C16/1045), Biotin conjugate, 0.1mg/mL used for?

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.