CD16 (C16/1045), CF647 conjugate, 0.1mg/mL

About this product

This antibody recognizes CD16 (FcÎ3RIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. Two similar genes represent CD16, CD16A (FcÎ3RIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcÎ3RIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC471045-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD162; CLA; Cutaneous lymphocyte associated antigen; P-selectin glycoprotein ligand 1; PSGL1; Selectin P ligand; SELPLG

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FCGR3A

Gene ID

2214

NCBI Gene ID

372679

UniProt

P08637

Swiss Prot

P08637

Host

Mouse

Species Reactivity

Human

Reactivity

CD16|Fc gamma receptor III

Immunogen

Recombinant human CD16 protein

Target Antigen

CD16 | Fc gamma receptor III

Clonality

Monoclonal

Clone

C16/1045

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (verified) |IF (verified)

Field of Research

Immunology

Positive Control

K-562 or U-937 cells. Lymph nodes and tonsils.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

50-80 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp W. et al. (eds) Leukocyte Typing IV, Oxford University Press, Oxford, 1989.

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647