CD28 (204.12), Biotin conjugate, 0.1mg/mL

About this product

Recognizes a glycoprotein of 44-88 kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB0164-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

T-cell-specific surface glycoprotein CD28; Tp44

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD28

Gene ID

940

NCBI Gene ID

591629

UniProt

P10747

Swiss Prot

P10747

Host

Mouse

Species Reactivity

Cow, Human, Mouse, Pig, Sheep

Reactivity

CD28

Immunogen

Stimulated human leucocytes

Target Antigen

CD28

Clonality

Monoclonal

Clone

204.12

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |Functional studies (published) |IF (verified)

Field of Research

Immunology

Positive Control

Jurkat cells or human lymph nodes and tonsils.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

44 kDa (monomer) ; 88 kDa (dimer)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Exp Immunol (2010) 162: 516-527. (receptor activation)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin