CD31 / PECAM-1 (158-2B3), CF647 conjugate, 0.1mg/mL

About this product

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470352-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

PECAM1

Gene ID

5175

NCBI Gene ID

376675 & 514412

UniProt

P16284

Swiss Prot

P16284

Host

Mouse

Species Reactivity

Human

Reactivity

CD31|PECAM-1

Immunogen

Stimulated human leukocytes (Workshop VI)

Target Antigen

CD31 | PECAM-1

Clonality

Monoclonal

Clone

158-2B3

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |Flow, surface (published) |IF (published) |IF (verified)

Field of Research

Angiogenesis, Cancer

Positive Control

Jurkat cells. Tonsil or Angiosarcoma.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

~100 kDa (endothelium) and ~130 kDa (platelets)

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Kishimoto, T, et al. (Eds) Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth International Workshop and Conference held in Kobe, Japan, 10-14 November 1996. J Immunol (1999) 163: 553-557. (IF; Flow, surface) Blood (2002) 100(10): 3597-3603. (IF)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647