CD34 (ICO-115), CF405S conjugate, 0.1mg/mL

About this product

This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens) . Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC040039-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD34

Gene ID

947

NCBI Gene ID

374990

UniProt

P28906

Swiss Prot

P28906

Host

Mouse

Species Reactivity

Human, Rat

Reactivity

CD34

Immunogen

Blast cells of a chronic myeloid leukemia patient

Target Antigen

CD34

Clonality

Monoclonal

Clone

ICO-115

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |Flow, surface (published) |IF|IHC, FFPE (published) |IHC, frozen (published) |WB (published)

Field of Research

Angiogenesis, Cancer

Positive Control

KG-1 cells, Tonsil, or Angiosarcoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

90-110 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Biol Regulators Homeostatic Agents (2001) 15(1):1-13. (IHC, FFPE; IHC, frozen; WB) Am J Physiol (2004) 287(6), H2670-H2676. (Flow)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF405S