CD35 / CR1 (E11), Biotin conjugate, 0.1mg/mL

About this product

Recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1) /CD35. This MAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1 activity. This MAb is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21 /CD35 /CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNCB0040-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

C3 binding protein, C3b/C4b receptor, C3BR, C4BR, Complement Component (3b/4b) receptor 1 including Knops blood group system, Complement receptor type 1, KN, Knops blood group antigen

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CR1

Gene ID

1378

NCBI Gene ID

334019

UniProt

P17927

Swiss Prot

P17927

Host

Mouse

Species Reactivity

Baboon, Cynomolgus, Human, Rhesus macaque

Reactivity

CD35|CR1

Immunogen

Intact human monocytes

Target Antigen

CD35 | CR1

Clonality

Monoclonal

Clone

E11

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |ELISA (published) |IHC, FFPE (verified) |WB (published)

Field of Research

Immunology

Positive Control

Follicular dendritic cells (FDC) in tonsil

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

210-220 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (1999) 162:7549-7554. (WB) Clin Exp Immunol (2005) 143: 274-280. (ELISA) Int Immunol (2013) 25(1): 25-33. (Flow, surface)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

Biotin