CD3e (RIV9), CF647 conjugate, 0.1mg/mL

About this product

Reacts with five invariable CD3 chains designated CD3y, CD3d, CD3e, CD3f, and CD3n with molecular weight ranging from 16-28 kDa. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470322-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD3 epsilon | CD3 TCR complex | CD3E | CD3e antigen epsilon polypeptide (TiT3 complex) | Leu-4 epsilon chain | T cell antigen receptor complex epsilon subunit of T3 | T-cell surface antigen T3 | T-cell surface glycoprotein CD3 epsilon chain | T3E | TCRE | TiT3 complex

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD3E

Gene ID

916

NCBI Gene ID

3003

UniProt

P07766

Swiss Prot

P07766

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Reactivity

CD3

Immunogen

Human peripheral lymphocytes

Target Antigen

CD3

Clonality

Monoclonal

Clone

RIV9

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published) |IHC, frozen (expected) |Flow, surface (verified) |IF (verified) |IP (published)

Field of Research

Immunology

Positive Control

Jurkat cells. Tonsil and lymph node

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

20 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Transplant Proc (1989) 21: 1026-7. (functional studies) Eur J Immunol (1990) 20(4): 943-945. (immunoprecipitation) Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989. Immunology (1994) 83: 457-464. (immunofluorescence, frozen tissue) Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995.

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG3, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647