CD44 Standard (156-3C11), 0.2mg/mL

About this product

Recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V) . Its epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s) . Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0460-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

LHR; BA-1; chondroitin sulfate proteoglycan 8 (CSPG8) ; Epican; Extracellular Matrix Receptor III (ECM III) ; GP90 Lymphocyte Homing Adhesion Receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; HAS; HUTCH I; Hyaluronate Receptor; Indian blood group; Inlu Related p80 Glycoprotein; Ly 24; MDU2; MDU3; MIC4; MUTCH I; Phagocytic Glycoprotein 1 (PGP-1)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD44

Gene ID

960

NCBI Gene ID

502328

UniProt

P16070

Swiss Prot

P16070

Host

Mouse

Species Reactivity

Baboon, Green monkey, Human

Reactivity

CD44 Standard

Immunogen

Stimulated human leukocytes

Target Antigen

CD44 Standard

Clonality

Monoclonal

Clone

156-3C11

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |IF (verified) |IHC, FFPE (verified) |WB (published)

Field of Research

Immunology

Positive Control

HeLa cells or paracortex in tonsil or lymph node.

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

80-95 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Oncotarget (2017) 8(54): 92143-92156. (flow, surface; immunofluorescence; western)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA