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Product details

CD45RO (UCHL-1), 1mg/mL

Catalog number BNUM0003-50

Price for BNUM0003-50

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Size / count 1x 50 µL

About this product

Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31) . The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNUM0003-50
Size / count
1x 50 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
PTPRC
Gene ID
5788
NCBI Gene ID
654514
UniProt
P08575
Swiss Prot
P08575
Host
Mouse
Species Reactivity
Chimpanzee, Human, Marmoset
Reactivity
CD45RO
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1) ; Recombinant human CD45RO protein (T200/797)
Target Antigen
CD45RO
Clonality
Monoclonal
Clone
UCHL-1
Type
Primary
Source
Animal
Origin
Animal
Applications
Functional studies (published) |Flow, surface (verified) |IF (verified) |IHC, FFPE (verified) |IP (published) |WB (published)
Field of Research
Immunology, Signal transduction
Positive Control
CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Stability
2 years
Concentration
1 mg/mL
Buffer
PBS, no BSA, no azide
Molecular Weight
180-185 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (1988) 64: 331-336. (immunoprecipitation) Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. J Biol Chem (1996) 271(2): 755-760. J Immunol (2005) 174: 2712-2719. (functional studies)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse)
Classification
PBS, no BSA, no sodium azide (CAS 26628-22-8)
Conjugate
Purified, BSA-free

Frequently Asked Questions

What is the catalog number for CD45RO (UCHL-1), 1mg/mL?

The catalog number for CD45RO (UCHL-1), 1mg/mL is BNUM0003-50.

What sizes are available for CD45RO (UCHL-1), 1mg/mL?

CD45RO (UCHL-1), 1mg/mL is available in the following size(s): 1x 50 µL.

Is CD45RO (UCHL-1), 1mg/mL in stock?

CD45RO (UCHL-1), 1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD45RO (UCHL-1), 1mg/mL?

CD45RO (UCHL-1), 1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD45RO (UCHL-1), 1mg/mL?

Estimated delivery time for CD45RO (UCHL-1), 1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD45RO (UCHL-1), 1mg/mL?

The safety data sheet for CD45RO (UCHL-1), 1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd0295061270361e0ffd44/msds/bnum0003-50.pdf.

What is CD45RO (UCHL-1), 1mg/mL used for?

Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31) . The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

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