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Product details

CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL

Catalog number BNC470003-100

Price for BNC470003-100

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Size / count 1x 100 µL

About this product

Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31) . The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC470003-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
PTPRC
Gene ID
5788
NCBI Gene ID
654514
UniProt
P08575
Swiss Prot
P08575
Host
Mouse
Species Reactivity
Chimpanzee, Human, Marmoset
Reactivity
CD45RO
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1) ; Recombinant human CD45RO protein (T200/797)
Target Antigen
CD45RO
Clonality
Monoclonal
Clone
UCHL-1
Type
Primary
Source
Animal
Origin
Animal
Applications
Functional studies (published) |Flow, surface (verified) |IF (verified) |IHC, FFPE (verified) |IP (published) |WB (published)
Field of Research
Immunology, Signal transduction
Positive Control
CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
180-185 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (1988) 64: 331-336. (immunoprecipitation) Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. J Biol Chem (1996) 271(2): 755-760. J Immunol (2005) 174: 2712-2719. (functional studies)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF647

Frequently Asked Questions

What is the catalog number for CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL?

The catalog number for CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL is BNC470003-100.

What sizes are available for CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL?

CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL in stock?

CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL?

CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL?

Estimated delivery time for CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL?

The safety data sheet for CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd02a6061270361e105dca/msds/bnc470003-100.pdf.

What is CD45RO (UCHL-1), CF647 conjugate, 0.1mg/mL used for?

Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31) . The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

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