CD47 (B6H12.2), 0.2mg/mL

About this product

This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0437-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Antigenic Surface Determinant Protein OA3; IAP; Integrin Associated Protein; Integrin Associated Signal Transducer; MER6; OA3; Protein MER6; Rh Related Antigen

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD47

Gene ID

961

NCBI Gene ID

446414

UniProt

Q08722

Swiss Prot

Q08722

Host

Mouse

Species Reactivity

Human, Mouse

Reactivity

CD47

Immunogen

Intact CD47 purified from placenta

Target Antigen

CD47

Clonality

Monoclonal

Clone

B6H12.2

Type

Primary

Source

Animal

Origin

Animal

Applications

IF (published) |Flow, surface (verified) |Functional studies (published) |IF (verified) |IP (published) |WB (published)

Field of Research

Cell adhesion, Hematology, Neuroscience, Signal transduction

Positive Control

HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors|MCF-7

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

~50 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications._x000D_ 1. J Biol Chem (2002) 277(12): 10028-10036. (functional studies)_x000D_ 2. Cell (2009) 138(2): 286-299. (functional studies)_x000D_ 3. J Immunol (2014) 193: 3914-3924. (immunofluorescence)_x000D_ 4. J Immunol (2015) 195: 661-671. (flow, surface; immunofluorescence; western)_x000D_ 5. Nat Med (2019) 25(4): 612-619. (immunoprecipitation)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA