CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL

Catalog number BNCB0177-100

Price for BNCB0177-100

Size / count 1x 100 µL

About this product

Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB0177-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: ICAMR; Intercellular adhesion molecule 3 (ICAM3)
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: ICAM3
Gene ID: 3385
NCBI Gene ID: 354563
UniProt: P32942
Swiss Prot: P32942
Host: Mouse
Species Reactivity: Human
Reactivity: CD50
Immunogen: Stimulated human leukocytes
Target Antigen: CD50
Clonality: Monoclonal
Clone: 101-1D2
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, surface (published) |Functional studies (published) |ELISA (published)
Field of Research: Immunology
Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 110-160 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press, 1989. Schlossman SF, et. al. Leucocyte Typing V, p1542-1547, 2011, Oxford Univ. Press, 1993. Eur J Immunol (1993) 23: 1508-1512. (IP, Flow) Eur J Immunol (1994) 24: 1377-1382. (functional studies) J of Immunol (1995) 154(6):3015-24. (ELISA)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL?

The catalog number for CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL is BNCB0177-100.

What sizes are available for CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL?

CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL in stock?

CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL?

CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL?

The safety data sheet for CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD50 (ICAM-3) (101-1D2), Biotin conjugate, 0.1mg/mL used for?

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.