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Product details

CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL

Catalog number BNC880177-100

Price for BNC880177-100

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Size / count 1x 100 µL

About this product

Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This MAb is excellent for staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNC880177-100
Size / count
1x 100 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
ICAMR; Intercellular adhesion molecule 3 (ICAM3)
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
ICAM3
Gene ID
3385
NCBI Gene ID
354563
UniProt
P32942
Swiss Prot
P32942
Host
Mouse
Species Reactivity
Human
Reactivity
CD50
Immunogen
Stimulated human leukocytes
Target Antigen
CD50
Clonality
Monoclonal
Clone
101-1D2
Type
Primary
Source
Animal
Origin
Animal
Applications
Flow, surface (published) |Functional studies (published) |ELISA (published)
Field of Research
Immunology
Positive Control
HL-60 or THP-1 cells. Lymph node and tonsil
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
110-160 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. Knapp, W. et. al. Leucocyte Typing IV, p541, 667-670, 1087, Oxford Univ. Press, 1989. Schlossman SF, et. al. Leucocyte Typing V, p1542-1547, 2011, Oxford Univ. Press, 1993. Eur J Immunol (1993) 23: 1508-1512. (IP, Flow) Eur J Immunol (1994) 24: 1377-1382. (functional studies) J of Immunol (1995) 154(6):3015-24. (ELISA)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Notes
Protect from light
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
CF488A

Frequently Asked Questions

What is the catalog number for CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL?

The catalog number for CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL is BNC880177-100.

What sizes are available for CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL?

CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL in stock?

CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL?

CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL?

Estimated delivery time for CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL?

The safety data sheet for CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD50 (ICAM-3) (101-1D2), CF488A conjugate, 0.1mg/mL used for?

Recognizes an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signal

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

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