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Product details

CD53 (161-2), Biotin conjugate, 0.1mg/mL

Catalog number BNCB0324-500

Price for BNCB0324-500

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Size / count 1x 500 µL

About this product

Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI; Code N-L033) . It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1) .Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number
BNCB0324-500
Size / count
1x 500 µL
Dry ice
No
Hazardous
No
Discontinued
No
CAS Number
9007-83-4
Synonyms
CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25) ; TSPAN25
UNSPSC
41116161
UNSPSC Description
Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name
CD53
Gene ID
963
NCBI Gene ID
443057
UniProt
P19397
Swiss Prot
P19397
Host
Mouse
Species Reactivity
Human
Reactivity
CD53
Immunogen
Stimulated human leukocytes
Target Antigen
CD53
Clonality
Monoclonal
Clone
161-2
Type
Primary
Source
Animal
Origin
Animal
Applications
Functional studies (published)
Field of Research
Immunology
Positive Control
Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
Stability
2 years
Concentration
0.1 mg/mL
Buffer
PBS, 0.1% BSA, 0.05% azide
Molecular Weight
33-55 kDa
References & Citations
Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (1999) 96(1): 35-47. (functional studies) Eur J Biochem (2002) 269: 1012-1021. (functional studies)
Shipping Conditions
RT (ND)
Storage Conditions
Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life
Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment
IgG2a, kappa
Species
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification
0.05% sodium azide (CAS 26628-22-8)
Conjugate
Biotin

Frequently Asked Questions

What is the catalog number for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

The catalog number for CD53 (161-2), Biotin conjugate, 0.1mg/mL is BNCB0324-500.

What sizes are available for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

CD53 (161-2), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD53 (161-2), Biotin conjugate, 0.1mg/mL in stock?

CD53 (161-2), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD53 (161-2), Biotin conjugate, 0.1mg/mL?

CD53 (161-2), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for CD53 (161-2), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

The safety data sheet for CD53 (161-2), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD53 (161-2), Biotin conjugate, 0.1mg/mL used for?

Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI; Code N-L033) . It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be th

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

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