CD53 (161-2), Biotin conjugate, 0.1mg/mL

Catalog number BNCB0324-100

Price for BNCB0324-100

Size / count 1x 100 µL

About this product

Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI; Code N-L033) . It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1) .Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB0324-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25) ; TSPAN25
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CD53
Gene ID: 963
NCBI Gene ID: 443057
UniProt: P19397
Swiss Prot: P19397
Host: Mouse
Species Reactivity: Human
Reactivity: CD53
Immunogen: Stimulated human leukocytes
Target Antigen: CD53
Clonality: Monoclonal
Clone: 161-2
Type: Primary
Source: Animal
Origin: Animal
Applications: Functional studies (published)
Field of Research: Immunology
Positive Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 33-55 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (1999) 96(1): 35-47. (functional studies) Eur J Biochem (2002) 269: 1012-1021. (functional studies)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

The catalog number for CD53 (161-2), Biotin conjugate, 0.1mg/mL is BNCB0324-100.

What sizes are available for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

CD53 (161-2), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is CD53 (161-2), Biotin conjugate, 0.1mg/mL in stock?

CD53 (161-2), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD53 (161-2), Biotin conjugate, 0.1mg/mL?

CD53 (161-2), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for CD53 (161-2), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD53 (161-2), Biotin conjugate, 0.1mg/mL?

The safety data sheet for CD53 (161-2), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD53 (161-2), Biotin conjugate, 0.1mg/mL used for?

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

Is this product species cross-reactive?

Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.