CD53 (161-2), CF594 conjugate, 0.1mg/mL

Catalog number BNC940324-500

Price for BNC940324-500

Size / count 1x 500 µL

About this product

Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI; Code N-L033) . It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1) .Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC940324-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CD53 glycoprotein; CD53 tetraspan antigen; Cell surface glycoprotein CD53; Leukocyte surface antigen CD53; Tetraspanin-25 (Tspan-25) ; TSPAN25
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CD53
Gene ID: 963
NCBI Gene ID: 443057
UniProt: P19397
Swiss Prot: P19397
Host: Mouse
Species Reactivity: Human
Reactivity: CD53
Immunogen: Stimulated human leukocytes
Target Antigen: CD53
Clonality: Monoclonal
Clone: 161-2
Type: Primary
Source: Animal
Origin: Animal
Applications: Functional studies (published)
Field of Research: Immunology
Positive Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 33-55 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (1999) 96(1): 35-47. (functional studies) Eur J Biochem (2002) 269: 1012-1021. (functional studies)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for CD53 (161-2), CF594 conjugate, 0.1mg/mL?

The catalog number for CD53 (161-2), CF594 conjugate, 0.1mg/mL is BNC940324-500.

What sizes are available for CD53 (161-2), CF594 conjugate, 0.1mg/mL?

CD53 (161-2), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD53 (161-2), CF594 conjugate, 0.1mg/mL in stock?

CD53 (161-2), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD53 (161-2), CF594 conjugate, 0.1mg/mL?

CD53 (161-2), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD53 (161-2), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for CD53 (161-2), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD53 (161-2), CF594 conjugate, 0.1mg/mL?

The safety data sheet for CD53 (161-2), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD53 (161-2), CF594 conjugate, 0.1mg/mL used for?

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.