CD59 (heavy chain of Protectin) (BRA-10G), 0.2mg/mL

About this product

Reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI) -anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH) . This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0181-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

20kDa homologous restriction factor (HRF20) ; Complement regulatory protein; Human leukocyte antigen MIC11; MAC-inhibitory protein (MACIP) ; Membrane attack complex inhibition factor (MACIF) ; Membrane inhibitor of reactive lysis; MIRL; MSK21; Protectin; T cell activating protein

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD59

Gene ID

966

NCBI Gene ID

278573; 709466; 710641

UniProt

P13987

Swiss Prot

P13987

Host

Mouse

Species Reactivity

Human

Reactivity

CD59

Immunogen

Human K562 tumor cells

Target Antigen

CD59

Clonality

Monoclonal

Clone

BRA-10G

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, surface (published) |IP (published) |WB (published)

Field of Research

Hematology, Immunology

Positive Control

Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

20 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Neoplasma (1992) 39(6),325-9. (surface Flow, IP) Diabetes (2002) 51(12), 3499-3504. (IP) Infection Immunity (2006) 74(2):1189-1195. (WB blot) Leukocyte Typing V (S F Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1476-1477.

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA