CD86 (BU63), 1mg/mL

About this product

Recognizes a protein of 70 kDa, which is identified as CD86. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4, a cancer immunotherapy target. It is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM0193-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

Activation B7-2 antigen|B lymphocyte activation antigen B72|CD28 antigen ligand 2|CD28LG2|CLS1|CTLA-4 counter-receptor B7.2|Early T-cell co-stimulatory molecule 1|ETC-1|FUN-1|LAB72|Ly-58|MB7-2|TS/A-2

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD86

Gene ID

942

NCBI Gene ID

171182

UniProt

P42081

Swiss Prot

P42081

Host

Mouse

Species Reactivity

Human, Mouse, Rat

Reactivity

CD86

Immunogen

ARH-77 (B-lymphoblastoid cell line)

Target Antigen

CD86

Clonality

Monoclonal

Clone

BU63

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |Flow, surface (published) |Functional studies (published) |IF (verified) |IHC, FFPE (verified)

Field of Research

Cancer immunotherapy/immune checkpoint, Immunology

Positive Control

293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil.

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

70 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Immunol (1998) 161: 6375-6382. (functional studies; Flow, surface)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free