CD95 (B-R18), 1mg/mL

About this product

MAb B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45 kDa, containing 8 kDa of N-glycosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) . CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUM0305-50

Size / count

1x 50 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

ALPS1A; Apoptosis APO1 antigen 1; Apoptosis-mediating surface antigen FAS; APT1; Delta Fas; Fas AMA; Fas cell surface death receptor; FAS1; FASL receptor; FASLG receptor; FASTM; Surface antigen APO1; Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

FAS

Gene ID

355

NCBI Gene ID

244139

UniProt

P25445

Swiss Prot

P25445

Host

Mouse

Species Reactivity

Human

Reactivity

CD95

Immunogen

Recombinant human CD95 protein

Target Antigen

CD95

Clonality

Monoclonal

Clone

B-R18

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published)

Field of Research

Apoptosis, Immunology

Positive Control

MCF-7 cells; hepatocellular or bladder carcinoma

Stability

2 years

Concentration

1 mg/mL

Buffer

PBS, no BSA, no azide

Molecular Weight

38-50 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Neuroimmunol (2000) 109: 211-220. (functional studies)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse)

Classification

PBS, no BSA, no sodium azide (CAS 26628-22-8)

Conjugate

Purified, BSA-free