CD95 (B-R18), CF405S conjugate, 0.1mg/mL

Catalog number BNC040305-500

Price for BNC040305-500

Size / count 1x 500 µL

About this product

MAb B-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45 kDa, containing 8 kDa of N-glycosidic-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) . CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC040305-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: ALPS1A; Apoptosis APO1 antigen 1; Apoptosis-mediating surface antigen FAS; APT1; Delta Fas; Fas AMA; Fas cell surface death receptor; FAS1; FASL receptor; FASLG receptor; FASTM; Surface antigen APO1; Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: FAS
Gene ID: 355
NCBI Gene ID: 244139
UniProt: P25445
Swiss Prot: P25445
Host: Mouse
Species Reactivity: Human
Reactivity: CD95
Immunogen: Recombinant human CD95 protein
Target Antigen: CD95
Clonality: Monoclonal
Clone: B-R18
Type: Primary
Source: Animal
Origin: Animal
Applications: Functional studies (published)
Field of Research: Apoptosis, Immunology
Positive Control: MCF-7 cells; hepatocellular or bladder carcinoma
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 38-50 kDa
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Neuroimmunol (2000) 109: 211-220. (functional studies)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF405S

Frequently Asked Questions

What is the catalog number for CD95 (B-R18), CF405S conjugate, 0.1mg/mL?

The catalog number for CD95 (B-R18), CF405S conjugate, 0.1mg/mL is BNC040305-500.

What sizes are available for CD95 (B-R18), CF405S conjugate, 0.1mg/mL?

CD95 (B-R18), CF405S conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is CD95 (B-R18), CF405S conjugate, 0.1mg/mL in stock?

CD95 (B-R18), CF405S conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures CD95 (B-R18), CF405S conjugate, 0.1mg/mL?

CD95 (B-R18), CF405S conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for CD95 (B-R18), CF405S conjugate, 0.1mg/mL?

Estimated delivery time for CD95 (B-R18), CF405S conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for CD95 (B-R18), CF405S conjugate, 0.1mg/mL?

The safety data sheet for CD95 (B-R18), CF405S conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is CD95 (B-R18), CF405S conjugate, 0.1mg/mL used for?

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.