Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL

Catalog number BNC940680-500

Price for BNC940680-500

Size / count 1x 500 µL

About this product

This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC940680-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CCNB1
Gene ID: 891
NCBI Gene ID: 23960
UniProt: P14635
Swiss Prot: P14635
Host: Mouse
Species Reactivity: Hamster, Human, Mouse
Reactivity: Cyclin B1
Immunogen: Hamster Cyclin B1 protein
Target Antigen: Cyclin B1
Clonality: Monoclonal
Clone: V92.1
Type: Primary
Source: Animal
Origin: Animal
Applications: Flow, intracellular (verified) |IF (verified)
Field of Research: Cell cycle
Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 55-62 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL?

The catalog number for Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL is BNC940680-500.

What sizes are available for Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL?

Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL in stock?

Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL?

Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL?

The safety data sheet for Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL is available here: https://cdn.gentaur.com/products/37/68dd0299061270361e101046/msds/bnc940680-500.pdf.

What is Cyclin B1 (V92.1), CF594 conjugate, 0.1mg/mL used for?

Do you offer bulk or custom quantities of this product?

Yes. Ordering two or more units, or combining this with other products, usually qualifies for a discount depending on quantity. Contact our sales team with your required volume for a custom quote.

Do you provide a Certificate of Analysis (CoA) for this product?

A Certificate of Analysis is available for most products upon request. Contact our support team with the lot number to obtain the relevant CoA.

Is this product suitable for live cell imaging?

Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.