CD22 / BL-CAM (B-Cell Marker) (BLCAM/1796), CF740 conjugate, 0.1mg/mL

About this product

This antibody recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM) . CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC741796-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

B-lymphocyte cell adhesion molecule (BL-CAM) ; B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2) ; SIGLEC2; T-cell surface antigen Leu-14

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD22

Gene ID

933

NCBI Gene ID

579691

UniProt

P20273

Swiss Prot

P20273

Host

Mouse

Species Reactivity

Human

Reactivity

BL-CAM|CD22

Immunogen

Recombinant fragment of human CD22 protein (around aa 52-178) (exact sequence is proprietary)

Target Antigen

BL-CAM | CD22

Clonality

Monoclonal

Clone

BLCAM/1796

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow (verified) |IF (verified) |WB (verified)

Field of Research

Immunology

Positive Control

Raji or Ramos cells (IF/FACS). Raji cell lysate (WB). Human tonsil, lymph node or spleen (IHC).

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

130-140 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), Bos taurus (bovine)

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF740