Cytokeratin 10 (LH2), CF647 conjugate, 0.1mg/mL

About this product

This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10) . CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC470674-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT10

Gene ID

3858

NCBI Gene ID

99936

UniProt

P13645

Swiss Prot

P13645

Host

Mouse

Species Reactivity

Human, Mouse

Reactivity

Cytokeratin 10

Immunogen

Skin extract of a human Psoriasis patient

Target Antigen

Cytokeratin 10

Clonality

Monoclonal

Clone

LH2

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified) |WB (verified)

Field of Research

Cancer, Cytoskeleton

Positive Control

A431, HeLa, MCF7 cells. Esophagus or tonsil.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

56.5 kDa

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF647