Cytokeratin 17 (E3), CF594 conjugate, 0.1mg/mL

About this product

Cytokeratin 17 (CK17) is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Antibody to CK17 is an excellent tool to distinguish myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. CK17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. It may be considered asepithelial stem cellmarker because CK17 Ab marks basal cell differentiation. CK17 is expressed in SCLC much higher than in LADC. Eighty-five percent of the triple negative breast carcinomas immunoreact with basal cytokeratins including anti-CK17. Also important is that cases of triple negative breast carcinoma with expression of CK17 show an aggressive clinical course. The histologic differentiation of ampullary cancer, intestinal vs. pancreatobiliary, is very important for treatment. Usually anti-CK17 and anti-MUC1 immunoreactivity represents pancreatobiliary subtype whereas anti-MUC2 and anti-CDX-2 positivity defines intestinal subtype. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC940158-500

Size / count

1x 500 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT17

Gene ID

3872

NCBI Gene ID

2785

UniProt

Q04695

Swiss Prot

Q04695

Host

Mouse

Species Reactivity

Cow, Goat, Human, Pig, Rat

Reactivity

Cytokeratin 17

Immunogen

The cytoskeletal fraction of rat colon epithelium

Target Antigen

Cytokeratin 17

Clonality

Monoclonal

Clone

E3

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular|IF (published) |IHC, FFPE (verified) |WB (verified)

Field of Research

Cancer, Cytoskeleton

Positive Control

T24 cells or Skin. Bladder or Cervix.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

46 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Sci (1989) 93: 419-426. (IF; WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2b, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF594