CD8A (Cytotoxic- & Suppressor T-Cell Marker) (UCHT4), CF740 conjugate, 0.1mg/mL

About this product

CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) . A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4 /CD8-. Anaplastic large cell lymphoma is usually CD4 and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen. This MAb binds to the CD8 alpha chain, at an epitope distinct from that recognized by UCHT14. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC741628-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CD8 antigen; alpha polypeptide (p32) ; T8/Leu-2 T-lymphocyte differentiation antigen; Ly3; LYT3; MAL; T-cell surface glycoprotein CD8 alpha chain

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

CD8A

Gene ID

925

NCBI Gene ID

85258

UniProt

P01732

Swiss Prot

P01732

Host

Mouse

Species Reactivity

Human

Reactivity

CD8A

Immunogen

Thymocytes followed by Sezary T cells.

Target Antigen

CD8A

Clonality

Monoclonal

Clone

UCHT4

Type

Primary

Source

Animal

Origin

Animal

Applications

Functional studies (published) |IF (published) |Flow, surface (verified) |IP (published)

Positive Control

Jurkat, HuT78 or hPBL. Tonsil.

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% rBSA, 0.05% azide

Molecular Weight

32 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. Eur J Immunol (2005) 35: 2896-2908. (functional studies) J Biol Chem (2007) 282(18): 13884-13894. (immunoprecipitation) Traffic (2015) 16(12): 1330-1341. (immunofluorescence)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG2a, kappa

Species

Mus musculus (mouse), Bos taurus (bovine)

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF740