Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL

Catalog number BNC941219-100

Price for BNC941219-100

Size / count 1x 100 µL

About this product

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19) . Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC941219-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT8
Gene ID: 3856
NCBI Gene ID: 533782; 708445
UniProt: P05787
Swiss Prot: P05787
Host: Mouse
Species Reactivity: Cow, Human, Pig, Rabbit, Sheep
Reactivity: Cytokeratin 8
Immunogen: Cytoskeletal preparation from HeLa cells
Target Antigen: Cytokeratin 8
Clonality: Monoclonal
Clone: B22.1
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Field of Research: Cancer, Cytoskeleton
Positive Control: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 52.5 kDa
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG2a, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF594

Frequently Asked Questions

What is the catalog number for Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL?

The catalog number for Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL is BNC941219-100.

What sizes are available for Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL?

Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL in stock?

Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL?

Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL?

Estimated delivery time for Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL?

The safety data sheet for Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL used for?

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.