Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL

Catalog number BNUB1221-500

Price for BNUB1221-500

Size / count 1x 500 µL

About this product

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNUB1221-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT8; KRT18
Gene ID: 3856 (CK8); 3875 (CK18)
NCBI Gene ID: 533782; 708445 (CK8); 406013 (CK18)
UniProt: P05787 (CK8); P05783 (CK18)
Swiss Prot: P05787 (CK8) ; P05783 (CK18)
Host: Mouse
Species Reactivity: Human
Reactivity: Cytokeratin 8/18
Immunogen: Cytoskeletal preparation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1)
Target Antigen: Cytokeratin 8/18
Clonality: Monoclonal
Clone: B22.1 B23.1
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Field of Research: Cancer, Cytoskeleton
Positive Control: MCF-7, HeLa, PMC-42 or A431 cells. Skin, Colon, lung or breast carcinoma
Stability: 2 years
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 52.5 kDa (CK8) ; 45 kDa (CK18)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG' s
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Purified, with BSA

Frequently Asked Questions

What is the catalog number for Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL?

The catalog number for Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL is BNUB1221-500.

What sizes are available for Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL?

Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL is available in the following size(s): 1x 500 µL.

Is Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL in stock?

Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL?

Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL?

Estimated delivery time for Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL?

The safety data sheet for Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin 8/18 (B22.1 + B23.1), 0.2mg/mL used for?

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.