Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL

Catalog number BNCB1221-500

Price for BNCB1221-500

Size / count 1x 500 µL

About this product

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB1221-500
Size / count: 1x 500 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT8; KRT18
Gene ID: 3856 (CK8); 3875 (CK18)
NCBI Gene ID: 533782; 708445 (CK8); 406013 (CK18)
UniProt: P05787 (CK8); P05783 (CK18)
Swiss Prot: P05787 (CK8) ; P05783 (CK18)
Host: Mouse
Species Reactivity: Human
Reactivity: Cytokeratin 8/18
Immunogen: Cytoskeletal preparation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1)
Target Antigen: Cytokeratin 8/18
Clonality: Monoclonal
Clone: B22.1 B23.1
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Field of Research: Cancer, Cytoskeleton
Positive Control: MCF-7, HeLa, PMC-42 or A431 cells. Skin, Colon, lung or breast carcinoma
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 52.5 kDa (CK8) ; 45 kDa (CK18)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG' s
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL?

The catalog number for Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL is BNCB1221-500.

What sizes are available for Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL?

Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 500 µL.

Is Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL in stock?

Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL?

Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL?

The safety data sheet for Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin 8/18 (B22.1 + B23.1), Biotin conjugate, 0.1mg/mL used for?

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.