Cytokeratin 8/18 (B22.1 + B23.1), CF543 conjugate, 0.1mg/mL

About this product

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNC431221-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT8; KRT18

Gene ID

3856 (CK8); 3875 (CK18)

NCBI Gene ID

533782; 708445 (CK8); 406013 (CK18)

UniProt

P05787 (CK8); P05783 (CK18)

Swiss Prot

P05787 (CK8) ; P05783 (CK18)

Host

Mouse

Species Reactivity

Human

Reactivity

Cytokeratin 8/18

Immunogen

Cytoskeletal preparation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1)

Target Antigen

Cytokeratin 8/18

Clonality

Monoclonal

Clone

B22.1 B23.1

Type

Primary

Source

Animal

Origin

Animal

Applications

IHC, FFPE (verified)

Field of Research

Cancer, Cytoskeleton

Positive Control

MCF-7, HeLa, PMC-42 or A431 cells. Skin, Colon, lung or breast carcinoma

Stability

2 years

Concentration

0.1 mg/mL

Buffer

PBS, 0.1% BSA, 0.05% azide

Molecular Weight

52.5 kDa (CK8) ; 45 kDa (CK18)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Notes

Protect from light

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG' s

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

0.05% sodium azide (CAS 26628-22-8)

Conjugate

CF543