Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL — image 1 of 2

Product details

Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL

Catalog number BNC040770-100

Price for BNC040770-100

Size / count 1x 100 µL

About this product

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNC040770-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT8; KRT18
Gene ID: 3856 (CK8); 3875 (CK18)
NCBI Gene ID: 533782; 708445 (CK8); 406013 (CK18)
UniProt: P05787 (CK8); P05783 (CK18)
Swiss Prot: P05787 (CK8) ; P05783 (CK18)
Host: Mouse
Species Reactivity: Human
Reactivity: Cytokeratin 8/18
Immunogen: Keratin preparation from a human carcinoma (C-43) ; PMC-42 human breast carcinoma cells (DC10)
Target Antigen: Cytokeratin 8/18
Clonality: Monoclonal
Clone: C-43 DC10
Type: Primary
Source: Animal
Origin: Animal
Applications: IHC, FFPE (verified)
Field of Research: Cancer, Cytoskeleton
Positive Control: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 52.5 kDa (CK8) ; 45 kDa (CK18)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Notes: Protect from light
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG' s
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: CF405S

Frequently Asked Questions

What is the catalog number for Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL?

The catalog number for Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL is BNC040770-100.

What sizes are available for Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL?

Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL in stock?

Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL?

Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL?

Estimated delivery time for Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL?

The safety data sheet for Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin 8/18 (C-43 + DC10), CF405S conjugate, 0.1mg/mL used for?

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.