Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL

Catalog number BNCB0034-100

Price for BNCB0034-100

Size / count 1x 100 µL

About this product

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number: BNCB0034-100
Size / count: 1x 100 µL
Dry ice: No
Hazardous: No
Discontinued: No
CAS Number: 9007-83-4
Synonyms: CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: KRT8; KRT18
Gene ID: 3856 (CK8); 3875 (CK18)
NCBI Gene ID: 533782; 708445 (CK8); 406013 (CK18)
UniProt: P05787 (CK8); P05783 (CK18)
Swiss Prot: P05787 (CK8) ; P05783 (CK18)
Host: Mouse
Species Reactivity: Cow, Human, Monkey, Pig, Sheep
Reactivity: Cytokeratin 8/18
Immunogen: Cytoskeleton preparation from HeLa cells
Target Antigen: Cytokeratin 8/18
Clonality: Monoclonal
Clone: C-51
Type: Primary
Source: Animal
Origin: Animal
Applications: IF (published) |IHC, FFPE (verified) |WB (published)
Field of Research: Cancer, Cytoskeleton
Positive Control: MCF-7 or A431 cells. Skin, Colon, lung or breast carcinoma
Stability: 2 years
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 52.5 kDa (CK8) ; 45 kDa (CK18)
References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Brain Pathol (1999) 9: 617-626. (IHC, FFPE) Br J Cancer (1999) 81(5): 769-773. (WB) Int J Cancer (2004) 111: 662-668. (WB) Cell (2014) 159(1): 163-175. (IF; IHC, FFPE)
Shipping Conditions: RT (ND)
Storage Conditions: Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: Guaranteed for at least 24 months from date of receipt when stored as recommended
Fragment: IgG1, kappa
Species: Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Classification: 0.05% sodium azide (CAS 26628-22-8)
Conjugate: Biotin

Frequently Asked Questions

What is the catalog number for Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL?

The catalog number for Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL is BNCB0034-100.

What sizes are available for Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL?

Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL is available in the following size(s): 1x 100 µL.

Is Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL in stock?

Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL?

Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL is manufactured by Biotium.

What is the estimated delivery time for Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL?

Estimated delivery time for Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL is approximately 15 business day(s), depending on your location.

Where can I find the safety data sheet (SDS/MSDS) for Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL?

The safety data sheet for Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL is available here: https://biotium.com/wp-content/uploads/2018/06/SDS-primary-antibodies.pdf.

What is Cytokeratin 8/18 (C-51), Biotin conjugate, 0.1mg/mL used for?

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.