Cytokeratin, HMW (AE-3), 0.2mg/mL

About this product

This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1) ; 64 kDa (CK3) ; 59 kDa (CK4) ; 58 kDa (CK5) ; 56 kDa (CK6) ; 52 kDa (CK8) . Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Specifications

Catalog number

BNUB0257-100

Size / count

1x 100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Synonyms

KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P) ; Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9

UNSPSC

41116161

UNSPSC Description

Primary and secondary antibodies for multiple methodology immunostaining detection application

Gene Name

KRT76

Gene ID

51350

NCBI Gene ID

654392

UniProt

Q01546

Swiss Prot

Q01546

Host

Mouse

Species Reactivity

Chicken, Cow, Dog, Human, Monkey, Mouse, Rabbit, Rat

Reactivity

Cytokeratin, HMW

Immunogen

Human epidermal keratin

Target Antigen

Cytokeratin, HMW

Clonality

Monoclonal

Clone

AE-3

Type

Primary

Source

Animal

Origin

Animal

Applications

Flow, intracellular|IF (published) |IHC, frozen (published) |IHC, FFPE (verified) |WB (published)

Field of Research

Cancer, Cytoskeleton

Positive Control

Epithelial cells, Skin or Adenocarcinomas

Stability

2 years

Concentration

0.2 mg/mL

Buffer

PBS, 0.05% BSA, 0.05% azide

Molecular Weight

52-67 kDa

References & Citations

Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1982) 95: 580-588. (IHC, frozen; IF; WB)

Shipping Conditions

RT (ND)

Storage Conditions

Stable at room temperature or 37°C (98°F) for 7 days.

Shelf Life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Fragment

IgG1, kappa

Species

Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.

Classification

PBS, 0.05% BSA, 0.05% sodium azide (CAS 26628-22-8)

Conjugate

Purified, with BSA